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Fig. 8. Detection of amph1 (A) and amph2 (B) in ECL cells by RT-PCR. Positive (brain) and negative controls are also shown. Owing to the inclusion of an alternative spliced site, two bands were detected for amph2 (B). Amplification of full-length amph2 from ECL cell cDNA produced several bands, implying the presence of multiple splice variants (C). MW, molecular weight markers. Overview of amph2 splice forms found in ECL cells (modified from Wigge et al., 1997) (D). Domain structure of the amph2 gene product. Binding sites for AP-2, clathrin and dynamin are marked. PRD, proline rich domain; SH3, src homology domain (E).





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