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Fig. 4. Nuclear PrP is associated with chromatin and aggregates when released from DNA. (A) Nuclease treatment of nuclei. Triton X-100-permeabilized nuclei from ScN2a cells were incubated at 37°C for 20 or 40 minutes with DNase I. After centrifugation, the supernatant (S) and the pellet (P) fractions were analyzed by immunoblotting with anti-PrP antibodies. (B) Effect of NaCl. Triton X-100-permeabilized nuclei from ScN2a cells. were incubated with the indicated concentration of NaCl and kept on ice for 15 minutes. The released or insoluble proteins following low speed centrifugation were analysed by immunoblotting with anti-PrP antibodies. (C) Effect of EDTA. Triton X-100-permeabilized nuclei from ScN2a cells were supplemented with 10 mM EDTA. After incubation at 25°C for 20 minutes, the samples were processed as in panel B.





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