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Fig. 1. Immunoblot detection of Cx43 and Cx26 expression in parental and stably transfected HeLa, HEK, and N2a cells. Triton X-100-soluble extracts prepared from HeLa cells (20 µg protein) or HEK cells (20 µg protein for Cx43 detection or 100 µg protein for Cx26 detection) or whole cell lysates prepared from N2a cells (75 µg protein) were resolved by SDS-PAGE, transferred to membranes and blotted with anti-Cx43 (top) or anti-Cx26 (bottom) antibodies. Migration of molecular mass markers is indicated to the left of the blot.





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