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Fig. 4. Single channel properties of gap junctions recorded from co-transfected N2aCx26/Cx43 cells compared to those from a heterotypic N2aCx26/Cx43-N2aCx43 cell pair. (A) Single channel properties of events in pairs of co-transfected N2aCx26/Cx43 cells. (Top panel) Multichannel recording during maintained Vj of 90 mV. Solid line represents zero current level; dashed lines represent discrete current steps indicative of opening and closing of channels. The current histograms indicate operation of at least two different channels with conductances of 135 pS and 73 pS (arrows) that resemble homotypic Cx26 and homotypic Cx43 channels, respectively. (Bottom panel) Multichannel recording during maintained Vj of 70 mV. The current histograms revealed a conductance of 139 pS (resembling homotypic Cx26 channels). (B) Bipolar pulse protocol (V1 and V2) and associated single channel currents (Ij) recorded from a N2aCx26/Cx43-N2aCx43 cell pair. Current histograms yielded similar conductances for positive and negative Vj: 69 pS and 72 pS for the main state and 16 pS-17pS for the residual state (dashed line) (which correspond to homotypic Cx43 channels).





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