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Fig. 7. Analysis of oligomeric association of Cx26 and/or Cx43 in Hela-Cx26 (A,B), HeLa-Cx43 (C), and HeLa-Cx43/Cx26 cells (D). (A) Immunoblot of fractions collected after centrifugation of Triton X-100 soluble extract from Hela-Cx26 cells through a 5-20% sucrose gradient. Prior to centrifugation, samples were incubated in buffer alone (top panel) or buffer containing 0.6% SDS to disrupt oligomers (bottom panel). The numbers under the lanes indicate the corresponding sucrose concentration as determined by refractometry. (B-D) Graphs of the densitometric values obtained after quantitation of Cx43 (triangles) or Cx26 (open and solid squares) immunoblots. For HeLa-Cx26 cells, the Triton-soluble material was untreated ({blacksquare}) or treated ({square}) with 0.6% SDS for 1 hour at 4°C before layering on the top of the sucrose gradient.





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