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Fig. 8. Affinity purification of Cx43 and potential associated proteins from N2a-Cx43(His)6/Cx26 cells. A Triton X-100-soluble extract was purified using a Ni-NTA resin and eluted with a series of increasing concentrations of L-histidine (as indicated). The presence and abundance of Cx43 and Cx26 were analyzed by immunoblotting in the cell extract, washes and eluted material using anti-Cx43 and anti-Cx26 antibodies.





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