QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Xenopus oocyte nuclei contain an extensive filamentous network associated with spherical bodies, a subset of which is recognized by antibodies specific for coilin. (A-E) Nuclear contents fixed and visualized by scanning electron microscopy (feSEM) after `peeling' away the nuclear envelope (A,B) and then applying either moderate (C) or strong (E) manual `spreading' forces. (D) Imaging by field emission electron microscopy (feSEM) of a gently opened oocyte nucleus; the folded-back nuclear envelope is visible at left, and intranuclear filaments associated with spherical bodies are seen at right. (F) Quantification of the diameters of spherical bodies (n=212 bodies). (G) Western blot of isolated Xenopus oocyte nuclei probed with anti-coilin antibodies. (H,I) Representative feSEM images of spherical bodies, fixed and labeled with anti-coilin antibodies (H) or no primary antibody (I), followed by incubation with secondary antibodies conjugated to 10 nm gold-particles (pseudo-colored yellow).

Return to article