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Fig. 6. Immuno-gold SEM of nuclear filaments with anti-actin antibodies. (A) Western blot of isolated Xenopus oocyte nuclei probed with anti-actin antibodies. (B,C) The filamentous network is specifically decorated by antibodies against actin, visualized using gold-conjugated secondary antibodies (pseudo-colored yellow). (D) Only the distal regions of NPC baskets (the filaments of which are marked by dotted lines), at and beyond the terminal ring, are labeled by anti-actin antibodies (arrows). (E) Control filaments treated with secondary gold-conjugated antibody only. Arrow indicates a single gold particle, seen in higher magnification in the inset.





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