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Fig. 9. Immuno-gold localization of protein 4.1 epitopes on the PLF network. (A) Western blot of manually isolated Xenopus oocyte nuclei probed with antibodies against protein 4.1 shows a major isoform of ~80 kDa, plus potential minor isoforms visible only upon longer exposure (data not shown). (B,C) FeSEM images of different PLFs showing protein 4.1 labeled with primary antibodies plus 10-nm-gold-labeled secondary antibodies. Gold particles were visualized directly in backscatter images (not shown), and are pseudo-colored yellow.





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