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Fig. 1. PLC ${zeta}$ -induced Ca²⁺ oscillations are cell cycle-dependent. PLC ${zeta}$ cRNA was injected into MII-arrested mouse eggs and at two different stages of the first cell cycle following parthenogenetic activation: interphase G1 and late G2. Eggs were microinjected with Fura-dextran and PLC ${zeta}$ cRNA. Intracellular Ca²⁺ was monitored by changes in fluorescence excitation ratio. Time zero indicates approximately the point at which PLC ${zeta}$ was microinjected. (a) A series of Ca²⁺ oscillations occurs during MII that ceases as the egg enters interphase (n=20). (b) MII eggs were activated with Sr²⁺ in the presence of cytochalsin D. 2 hours after pronuclear formation the eggs were injected with Fura-dextran and PLC ${zeta}$ . No Ca²⁺ transients were detected in these parthenotes (n=20). (c) Parthenogentically activated eggs were cultured until interphase (G2), then 2-6 hours before NEB was due to occur embryos were injected with Fura-dextran and PLC ${zeta}$ cRNA (n=15). Ca²⁺ oscillations again occur.

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