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Fig. 3. PLC ${zeta}$ localises to the pronuclei. MII eggs were injected with PLC ${zeta}$ tagged with a c-Myc epitope and cultured in the presence of cytochalsin D until pronuclear formation, then they were fixed and stained using the c-Myc (9E10) monoclonal antibody and the anti-mouse Alexa Fluor® 488 secondary antibody. Control eggs were activated with untagged PLC ${zeta}$ . Confocal and brightfield images were simultaneously obtained. The black arrows in brightfield images indicate the nucleoli. Though not easily visible, the nuclear membrane surrounds the nucleolus. The control embryo shows non-specific binding. Eggs activated with Myc-PLC ${zeta}$ show a striking localisation to the nucleoplasm of each pronuclei. Scale bar: 10 µm.

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