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Fig. 5. Preventing PLC ${zeta}$ nuclear localisation permits Ca²⁺ oscillations during interphase. MII eggs were activated with Sr²⁺ in the presence of cytochalsin D. 2 hours after pronuclear formation the eggs were injected with Fura-dextran and Myc-PLC ${zeta}$ . (a) No Ca²⁺ transients were detected in these parthenotes (n=20). (b) Parallel parthenotes were also injected with WGA to block nuclear transport. In these embryos Myc-PLC ${zeta}$ triggered Ca²⁺ oscillations within 1 hour (n=20). Embryos were fixed and stained at the end of the experiment (as in Fig. 2) demonstrating that blocking nuclear pores with WGA, prevents import of PLC ${zeta}$ into the pronuclei (aii,bii). (c) Parthenotes were also injected with Myc-PLC ${zeta}$ ^K377E (n=10). As with inhibiting nuclear import, preventing nuclear localisation of PLC ${zeta}$ through mutation of the NLS permitted Ca²⁺ oscillations during interphase. Such Ca²⁺ oscillations were not observed in parallel controls (n=10).

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