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Fig. 6. The eIF4GI nuclear localisation signal is sufficient to direct a change in the subcellular localisation of eGFP. (A) Schematic representation of eIF4GI outlining the individual sequences used for expression in this figure. (B) Total cell extracts from HeLa cells expressing eGFP fusion proteins were resolved by SDS-PAGE. Expressed proteins were visualised by immunoblotting using a monoclonal antibody raised against eGFP; molecular mass markers are shown on the left. (C) HeLa cells expressing eGFP fusion proteins were grown on glass coverslips and fixed with paraformaldehyde. eGFP was visualised directly using the appropriate fluorescence filters. Scale bars: 20 µm.





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