|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 1. p38 MAPK is involved in PDGF-induced actin polymerization. Determination of F-actin/G-actin ratio. Suspended SMC were incubated for 30 minutes with 25 µM SB203580 or vehicle and then plated on fibronectin-coated wells for 3 hours in the presence or absence of PDGF (10 ng/ml). Fixed SMC were labeled with TRITC-phalloidin (2 µM) or Alexa 594-DNase I (3 µM). Fluorescence intensities were quantified and relative ratios of F- to G-actin fluorescence were then determined. Data represent the mean±s.e.m. of three independent experiments, carried out in triplicate. **Statistically significant according to Student's t-test: P<0.01.
|
