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Fig. 2. p38 MAPK is involved in PDGF-induced chemotaxis and cytoskeletal reorganization. (A) Migration assay. Pre-labeled suspended SMC were incubated for 30 minutes with 25 µM SB203580 (SB, closed circles and closed squares), or vehicle (open circles and open squares) and subjected to the migration assay in the presence (circles) or absence (squares) of PDGF (10 ng/ml) in the lower chamber. The fluorescence of migrating cells was determined at different times. Data represent the mean±s.e.m. of three independent experiments, carried out in triplicate. (B) Suspended SMC were incubated for 30 minutes with vehicle, 25 µM SB203580 or 25 µM SB202474 and then plated on fibronectin-coated coverslips for 3 hours in the presence or absence of PDGF (10 ng/ml). SMC were fixed and F-actin was visualized with Alexa 488-phalloidin. Bar, 10 µm. These results are representative of three independent experiments.
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