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Fig. 7. Hsp27 localization at the leading edge requires free barbed ends. Suspended SMC were plated on fibronectin-coated coverslips for 3 hours in the presence of PDGF (10 ng/ml) and treated with 150 nM cytochalasin D (CD, closed squares), 80 nM latrunculin B (LB, closed triangles) or vehicle (open circles), for the last 30 minutes of spreading. (A) Fixed SMC were double-labeled for F-actin and Hsp27. Bar, 5 µm. These results are representative of four independent experiments. (B) Distributions of F-actin and Hsp27 at the leading edge (0.9 µm from the membrane). Results are expressed as a percentage of the total fluorescence for each fluorophore. Data represent the mean±s.e.m. o f four independent experiments, carried out on at least ten cells in each experimental condition.





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