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Fig. 1. Formation of canal-like structures in primmorphs from S. domuncula. (A) Dissociated cells were obtained and (B) transferred to seawater-based medium; after 3 days aggregates with a rough surface were formed. (C) After a prolongation of the incubation for 6 days round primmorphs with a smooth surface (surrounded by choanocytes) were formed. (D) A subsequent transfer of the primmorphs onto galectin-coated culture dishes resulted, after six days (total incubation period of 12 days), in the formation of canal-like structures. (E) A higher magnification of the structures. (F) A primmorph that was cultured for the complete incubation period of 12 days in uncoated culture dishes; these structures remained in the non-attached state and were roughly spherical. Original magnifications: (A) x50; (B,C,F) x10; (D) x5; (E) x50.





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