spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Expression of a part of the S. domuncula SDADAMTS sequence including the CD36 binding motif. As outlined under Materials and Methods the fragment was subcloned into the bacterial expression vector pET41a and expression was performed in E. coli BL21 using IPTG. Lysates from non-induced (–IPTG; lane a) as well as from induced bacterial cultures (+IPTG; lane b) were prepared and analyzed using 15% polyacrylamide gel containing NaDodSO4; the gel was stained with Coomassie Brilliant Blue. The protein extract containing the recombinant SDADAMTS was purified/enriched by affinity chromatography (lane c).





Right arrow Return to article