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Fig. 1. Expression of ER proteins in response to CPA treatment. Protein extracts from untreated and CPA-treated soybean cells were analyzed by western blotting using anti-BiP (A), anti-CRT (B) and anti-caspase 12 (C) antibodies. Quantitative analyses were carried out as described in Materials and Methods and are represented by histograms: white bars, untreated cells (Co); grey bars, CPA-treated cells (CPA). In C the quantitative data refers to the procaspase 12-like (proc 12) protein band. Cells were treated with 50 µM CPA for 24 hours. The values for untreated cells were taken as 100%. Data are mean±s.d. of three independent experiments. Statistically significant at *P<0.05 and **P<0.005.





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