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Fig. 1. Activity of HET-s deletion constructs. (A) On the left, various deletion constructs of HET-s are depicted. Numberings correspond to amino acid positions. The prion-forming domain is shown in gray. For each construct, the abilities to propagate [Het-s], to produce a barrage reaction toward het-S (incompatibility function) and to aggregate into amyloid fibrils in vitro are listed. For the 218-289 construct, the results are for the 218-289 peptide fused to GFP. (B) Barrage test with various HET-s deletion constructs: a het-S tester strain (central line) was confronted on solid medium with strains bearing various HET-s deletion constructs. Confrontation between incompatible strains leads to the formation of a dark, dense contact line termed the barrage (black, arrowed). Compatible strains display normal clear contact lines (white arrowed). The strains that do not produce a barrage reaction to het-S are grouped on the top line; the strains producing a barrage reaction to het-S are on the bottom line. Notice the attenuated barrage reaction obtained with the het-s(218-289)GFP construct. (C) Strains were cultivated for 5 days on solid medium. Notice that expression of HET-s(157-289) in a het-S background strongly affects growth, whereas strains expressing HET-s(157-289) in a het-s or het-s background display normal growth. (D) Microscopic examination of a transformant expressing HET-s(157-289) in a het-S background and of an untransformed het-S control strain. Notice the extensive vacuolization of the hyphae in strains expressing HET-s(157-289). Scale bar, 4 µm.

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