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Fig. 2. Influence of L{gamma}f on deposition of basement membrane (BM) components shown by indirect immunofluorescence (IIF) at days 10 (A-F), 14 (G-K) and 19 (L,M). Compared to controls (left column) treated epithelia (right column) have lost or dramatically reduced nidogen (A,B), BM laminin (C,D and G,H) and perlecan (L,M). For laminin detection either antibodies against the laminin {gamma}1 chain (C,D) or laminin from EHS tumours (G,H) were employed. By contrast, type IV collagen (E,F and I,K) and laminin-5 (see Figs 3, 4) are only mildly affected by the treatment. Scale bars: 50 µm.





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