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Fig. 7. Mitochondrial DNA nucleoids are mobile within {rho}+ mitochondria. Stably transfected human 143B cells expressing GFPOM were co-plated with human HeLa cells that had been pre-incubated with BrdU. Co-plated cells were analyzed without further treatment (A), or 12 hours (B,C) or 24 hours (D) after PEG-mediated cell fusion. (A) The nuclear and mitochondrial DNA of untransfected HeLa cells is labeled with BrdU, and GFPOM-transfected cells are devoid of BrdU-labeling. (B-D) The polykaryons derived from the fusion of HeLa and 143B cells are positive for GFPOM and BrdU. The GFPOM-protein is distributed throughout the entire mitochondrial network (B-D). Twelve hours after fusion, BrdU-labeled nucleoids are absent from some regions of the mitochondrial network (B) or distribute to the entire mitochondrial compartment (C). Twenty-four hours after fusion, BrdU-labeled nucleoids are seen throughout the entire mitochondrial compartment. The outlines of the polykaryons are depicted with a white line.





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