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Fig. 3. Rapamycin induces the localization of Rab7T22N to autophagic vacuoles. CHO cells expressing EGFP-Rab7T22N were incubated at 37°C for 2 hours in {alpha}-MEM (control cells) or EBSS medium (starved cells) in the presence of 50 ng/µl rapamycin. Cells were briefly washed with PBS, labeled with MDC and immediately analyzed by fluorescence microscopy as described in Fig. 1.





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