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Fig. 7. Overexpression of Rab7 T22N suppresses the autophagic degradation of labeled long-lived proteins. CHO cells overexpressing EGFP-Rab7 wt, and the mutants Q67L or T22N, or the vector alone (EGFP) were labeled for 24 hours in medium containing 1 µCi/ml [3H]leucine (see Materials and Methods). Both the trichloroacetic acid (TCA)-precipitable radioactivity of the cell monolayers and the TCA-soluble radioactivity present in the medium were measured. Leucine released (in the TCA-soluble supernatant) was calculated as a percentage of the total cell radioactivity. Results were expressed as the mean±s.e.m. **Significantly different from the vector under starvation conditions P<0.001 (compared with ANOVA-1 and Turkey-Kramer test).





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