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Fig. 6. Cx37 is recruited to sites where seeded Cx37-positive oocytes contact granulosa cells. Wild-type (B) or Cx37-deficient (C) denuded oocytes were seeded onto cultured granulosa cell monolayers for 4 hours, fixed, and immunofluorescently labelled for Cx37 (anti-Cx37, red) and Cx43 (anti-Cx43, green). The oocytes became detached from the granulosa cells during fixation. Wild-type oocytes recruited Cx37 (arrows) to sites of oocyte-granulosa cell contact (B), whereas granulosa cells contacting Cx37-deficient oocytes (C) or not in contact with oocytes (control, A) exhibited little or no localized Cx37. RT-PCR using Cx37 specific primers was performed with RNA isolated from wild-type mouse lung, lung from Cx37-deficient mice, and cultured mouse granulosa cells (D). Amplicons of the predicted size were found in wild-type lung and granulosa cells, but were absent from lungs obtained from Cx37-deficient mice and when RNA templates were absent (negative control). Bar=10 µm.
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