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Fig. 7. Inhibition of HUVEC proliferation following transduction with either Ad-shChM-I or Ad-shTeM. HUVECs transduced with either Ad-EGFP, Ad-shChM-I or Ad-shTeM were plated into 96-well plates. The cells were starved in 0.5% FBS-containing {alpha}MEM for 6 hours and then incubated with VEGF (20 ng/ml) for 12 hours. The rate of DNA synthesis was evaluated by BrdU incorporation followed by measurement of BrdU ELISA chemiluminescence. A MAP kinase inhibitor, PD98059 (50 µM), was used as a negative control. Data are mean±s.d. of triplicate measurements.





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