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Fig. 8. Inhibition of migration in HUVECs transduced with Ad-shChM-I or Ad-shTeM. (A) Cell migration was evaluated using a modified Boyden chamber assay with vitronectin-coated filters. Representative images of HUVECs migrating to the lower side of the membranes in the presence of VEGF (20 ng/ml) are shown. Bar, 50 µm. (B) The number of cells migrating to the lower side of the membranes was measured. Data are mean±s.d. of triplicate measurements. *P<0.01 versus wild-type HUVECs.





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