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Fig. 9. Inhibition of cell adhesion and spreading via vitronectin in HUVECs transduced with Ad-shChM-I or Ad-shTeM. (A) Both adenovirus-transduced and wild-type HUVECs were harvested with trypsin/EDTA and suspended in culture medium for 20 minutes. The cells were re-suspended in 
MEM containing 0.1% BSA at a density of 5x105 cells/ml and seeded on 96-well culture plates coated with type I collagen, fibronectin or vitronectin. After incubation for 1 hour at 37°C, unattached cells were removed by gentle washing with PBS. Cellular morphologies of adherent cells were observed and photographed by fluorescent microscopy. Bar, 100 µm. (B) For quantification of adhesion, the adherent cells were stained with crystal violet. The crystal violet stain was eluted with 0.1 M sodium citrate (pH 4.2) in 50% ethanol and the number of adherent cells in each well was evaluated by the measurement of absorbance at 596 nm. Data are means±s.d. of triplicate assays.
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