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Fig. 9. The VEGFR-3 (+) subpopulation of BAE cells transmigrates more efficiently in response to VEGF-C_NC. BAE cells were induced to transmigrate as described in Fig. 8. Non-migrating cells were removed from the upper aspect of the membrane by scraping and the transmigrated cells were released from the lower surface with protease-free cell dissociation buffer. Expression of VEGFR-3 was assayed by FACS as described in Fig. 3, and the RFI of each peak is shown in yellow. Each panel shows the staining in the absence (green) and presence (blue) of hF4-3C5. The cells that did not migrate in response to VEGF-C_NC (non-migrated) exhibit the bimodal pattern of VEGFR-3 expression with about 50% of positive cells. The BAE cells that transmigrated in response to VEGF-C_NC (Migrated) are approximately 90% positive for VEGFR-3. This shift is reversed seen when the BAE cells transmigrate in response to VEGF₁₆₅ with the majority of cells being negative for VEGFR-3.

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