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Fig. 3. Antimycin, an inhibitor of the electron transport chain, doubles retrograde transport. (A) Kymograph of distribution and movements of Mitotracker-labeled mitochondria. Transport of the mitochondria was observed for 5-10 minutes for the pre-drug, early drug, late drug and washout application conditions as delineated by the horizontal green lines. The position and time of drug application (100 µM antimycin and Lucifer Yellow in DMEM) is shown in blue. The length of the axon was also divided into three sections with reference to the position of drug application (cell-body side, CB; position of drug application, drug; growth-cone side, GC). (B) Hand-drawn traces of anterograde (blue) and retrograde (red) movements used to quantify the mitochondrial transport. (C-F) Normalized anterograde and retrograde transport. Error bars are the normalized 95% confidence intervals (n=5 experiments). (D) There is significant transient increase in retrograde transport but no effect on anterograde transport. (E) After 5 minutes, retrograde transport returns to baseline levels.





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