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Fig. 7. The direction of mitochondrial transport is correlated with mitochondrial potential as assayed with JC-1 and Mitotracker FM-green. (A) Hand-drawn traces of anterograde and retrograde mitochondrial movements from the middle of an axon. Red and green lines represent the movements of mitochondria with a correspondingly high or low ratio of red/green staining. The arrow and triangle point to two mitochondrial traces that are exceptions to the correlation between potential and direction of transport. The vertical bar equals 1 minute and the horizontal bar equals 10 µm. (B) Color-inverted kymograph of red channel showing movements of mitochondria with high potential as assayed by JC-1. The circle indicates a mitochondrion undergoing a decrease in potential. (C) Color-inverted kymograph of green channel showing movements of JC-1/Mitotracker-FM-green-stained mitochondria. (D) The first frame of the time-lapse sequence shows the mixed polarity of mitochondria along the axon. (E) Overlay of red and green channels. (F) The last frame of the time-lapse sequence. (G) Hand-drawn traces of mitochondrial movements close to the growth cone. (H) Color-inverted kymograph of red channel. The double circle illustrates accumulation of mitochondria with high potential at the growth cone. (I) Color-inverted kymograph of green channel. (J) The first frame of the time-lapse sequence. (K) Overlay of red and green channels. (L) The last frame of the time-lapse sequence.





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