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Fig. 5. RT-PCR examination for RyR1 to 3. (A) Control amplicons for RyR1 to 3 and GAPDH extracted from brain, heart and skeletal muscle. Amplification, 35 cycles. (B) RT-PCR examinations of NTC (no template control) and RNA samples obtained from c-Kit-immunopositive cells and smooth muscle cells. Amplification, 45 cycles. (C) RT-PCR examinations for FKBP12 and FKBP12.6. Amplification, 45 cycles. In B, expression of c-kit was also examined. The numbers in the right of each gel indicate the size marker (bp).





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