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Fig. 2. MDCK-Snail and MDCK-E47 cells exhibit invasive and migratory abilities in in vivo transplantation assays. (A) low power and (B) high power images of MDCK-CMV (CMV) (a,d), MDCK-Snail (Snail) (b,e) and MDCK-E47 (E47) cells (c,f) grown on collagen type I gel, transplanted onto the backs of nude mice and allowed to grow for 10 days. Cryostat sections of each type of transplant were analysed by histology (a-c) and double immunofluorescence for E-cadherin (red) and vimentin (green) (d-f); nuclei were stained with DAPI (blue). (A) The lower limit of the collagen gel in contact with the host stroma is indicated by dashed black (a-c) and yellow lines (d-f). MDCK-CMV cells form an organized epithelial multilayer with cyst like structures without infiltration into the collagen gel (black arrowheads in a), whereas MDCK-Snail cells are clearly infiltrating (black arrowheads in b). Note also vimentin-positive cells apparently migrating from the host stroma into the collagen gel in MDCK-Snail and MDCK-E47 transplants (red arrows in b, c, e and f), in contrast to the complete absence of cells in the lower part of the collagen gel in MDCK-CMV transplants (a,d). Bar, 50 µm. (B) High power images of the upper part of the indicated 10-day transplants showing the histology (a-c) and immunofluorescence analyses for E-cadherin (red) and vimentin (green) (d-f); nuclei were stained with DAPI (blue). Note the absence of E-cadherin and expression of vimentin in all cell layers of MDCK-Snail (e) and MDCK-E47 (f) transplants. Bar, 50 µm.
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