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Fig. 3. Twenty day-transplants of MDCK-Snail and MDCK-E47 cells exhibit a high infiltration potential. Transplants of the indicated cells lines were allowed to grow on the backs of nude mice for 20 days and subsequently either (A) fixed in formaldehyde and processed for histology or (B) frozen in OCT and subjected to immunofluorescence staining after cryostat sectioning. (A) MDCK-CMV (CMV) (a,d) cells form a multilayer of highly differentiated epithelial cells with a clear basal membrane delimitation from the remaining collagen gel (dashed line in a, and yellow arrow in d). MDCK-Snail (b,e) and MDCK-E47 (c,f) cells fully infiltrate the collagen gel and adipose and muscle stromal tissues; the dashed lines in b and c indicate the hypothetical limit of the collagen gel. Panels d to f show magnified images of the lower part of the transplants. See also blood vessels in the lower part of MDCK-E47 transplants (c,f). Bar, 50 µm. (B) MDCK-CMV transplants maintain E-cadherin expression and absence of vimentin (a,d) in all cell layers, while no expression of E-cadherin and homogeneous expression of vimentin is observed in all cells of MDCK-E47 transplants (c,f). Invading cells of MDCK-Snail transplants do not express E-cadherin and are vimentin positive, while the upper non invading layers re-express E-cadherin and have lost vimentin (b,e). Nuclei were stained with DAPI (blue). Bar, 50 µm.

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