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Fig. 4. MDCK-Snail and MDCK-E47 cells induce an angiogenic response in the host stromal tissue. Cryostat sections of 10-day transplants of MDCK-CMV (a,d,g), MDCK-Snail (b,e,h) and MDCK-E47 (c,f,i) cells were subjected to histological (a-c) and immunofluorescence analysis (d-i) for CD31 (red) and vimentin (green). Yellow arrows indicate the border between the collagen gel and host stromal tissue, red arrows indicate the upper limit of CD31-positive cells. (g-i) Magnified images of CD31 positive cells (indicated as insets in d-f) are shown; white arrows indicate blood vessels. Bars, 50 µm.





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