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Fig. 5. Angiogenic response of 20-day transplants. MDCK-CMV (CMV) (a,d), MDCK-Snail (b,e) and MDCK-E47 (c,f) transplants grown for 20 days on the backs of nude mice were fixed in formaldehyde and processed for histological analysis. (a-d) Images corresponding to the lower part of the transplants; yellow arrows indicate the limit within the basal membrane and the remaining collagen gel (a) or the hypothetical edge of the gel (b,c). (d-f) Magnified images of the lower (d) and upper part (e,f) of the transplants. Blood vessels (indicated by blue arrows) were detected in the stromal region of CMV transplants (a,d) while they extend all over the surface of Snail (b,e) and E47 (c,f) transplants. Bars, 50 µm.





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