|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Effect of PKC downregulation or PKC inhibition on ALCAM-affinity. Soluble ALCAM-Fc-binding assay with KG1 cells (A) and K562-ALCAM (B) either cultured for 16 hours with (
) or without (
) 50 nM PMA or pre-incubated for 10 minutes at 37°C with chelerythrine chloride (5 µM, 
) or myristoylated PKC pseudosubstrate (100 µM, 
). Cells are incubated with increasing concentrations of soluble ALCAM-Fc and the percentage of cells that have bound ligand is determined by flow cytometry. Data are representative of three independent experiments.
|
