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Fig. 6. Cellular distribution of TBP mRNA transcribed from the intron-containing gene (A) or intron-less cDNA (B) in ptr11-1 and nuc1. Wild-type 972, ptr11-1 or nuc1 cells harboring the TBP-expressing plasmid was cultured at 26°C without thiamine for 16 hours, then maintained at 26°C or shifted to 37°C for 6 hours. The cells were subsequently subjected to in situ hybridization with the Cy3-labeled TBP probe specific for the mRNA expressed from the plasmid. The first and third columns show the intracellular distribution of the TBP mRNA and the second and fourth columns show cells stained with DAPI in the corresponding fields.
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