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Fig. 7. spMex67p-GFP accumulates in the nucleolar region in the heat-shocked cells. (A) The wild-type S. pombe cells expressing spMex67p-GFP from the weakest nmt1 promoter were grown at 30°C and either maintained at 30°C (left panels) or shifted to 42°C for 3 or 30 minutes (middle and right panels). After staining with Hoechst 33342, the distribution of spMex67p-GFP was observed with a fluorescence microscope. Insets in the left panels show enlarged pictures of the nucleus. In merged images, red denotes DNA and green denotes spMex67p-GFP. (B) Wild-type cells expressing Cut15p-GFP using the native cut15 promoter were grown at 30°C (left panel) or heat shocked at 42°C for 30 min (right panel). Intracellular distribution of Cut15p-GFP was not changed after the heat shock treatment, whereas spMex67p-GFP accumulates rapidly in the nucleolar region under the same conditions.





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