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Fig. 1. D2D3_88-274 inhibits MCP-1 induced monocyte migration. (A) Human monocytes were obtained from the peripheral blood of healthy volunteers using Ficoll and Percoll gradients. Cell migration in response to the indicated concentration of D2D3_88-274 ( ${blacksquare}$ ), ${Delta}$ D2D3 ( ${diamond}$ ) and D1 ( ${diamondsuit}$ ) was determined in a Boyden chamber as described in Materials and Methods. As control, cells were allowed to migrate in response to 2 nM MCP-1 (). The number of cells migrating in the absence of chemoattractant was taken as 100%. (B) Monocytes or (C) THP-1 cells were left either untreated or stimulated for 15 minutes with D2D3_88-274 ( ${blacksquare}$ ), D2D3_84-274 ( ${square}$ ), ${Delta}$ D2D3 ( ${diamond}$ ) and D1 ( ${diamondsuit}$ ) as indicated. Thereafter, the ability of the cells to migrate in response to 2 nM MCP-1 was determined. The number of cells migrating towards MCP-1 was taken as 100% and the values of cells migrating after pretreatment with D2D3_88-274 were calculated relative to this. Each point represents the mean±s.d. of three independent experiments.

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