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Fig. 3. D2D388-274 inhibits ß2-integrin-dependent monocyte migration. (A) THP-1 cells were pretreated for 15 minutes at 37°C with increasing concentrations of D1 (
), 
D2D3 (gray ), D2D388-274 (
) or D2D388-274 (
). (B, C) THP-1 cells were pretreated for 15 minutes at 37°C with increasing concentrations of MMK-1 and ATF. After pretreatment, cells were tested for their ability to migrate in response to fMLP (A,B,C), RANTES (A) or MCP-1 (B,C).
The number of cells migrating towards the different chemokines was taken as 100%, and the values of cells migrating after pretreatment with pretreating reagents were calculated relative to this. (D) THP-1 cells were incubated with either neutralizing anti-ß2 mAb or with an isotype-matched control-mAb. Thereafter the cells were allowed to migrate in response to MCP-1 (2 nM). The data in A, B, C and D represent the mean±s.d. of three experiments. Each experiment was performed in triplicate for each stimulus.
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