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Fig. 3. SSC frequency facilitation induced by RA requires continued presence of the factor. (A) The continuous trace depicts the inward membrane current recorded from an innervated muscle cell in Xenopus culture (filtered at 10 kHz). The arrow marks the time of addition of RA to the culture medium and RA was removed by the substitution of the culture medium with Ringer's solution at the time indicated by `wash'. Scale bars, 2 nA, 20 seconds and 1 nA, 50 milliseconds. (B) Changes in the SSC frequency (normalized to control frequency) with time during the addition and removal of 30 µM RA. The increased SSC frequency declined to control level within 5 minutes of washout of RA.





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