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Fig. 1. cAMP-mediated translocation of PHCrac-GFP. Dictyostelium cells were stimulated at t=0 seconds in a perfusion chamber with a homogeneous cAMP concentration of 1 µM or 1 nM cAMP. Confocal images were taken at the times indicated. PHCrac-GFP is in the cytosol before cAMP stimulation, and is translocated to the entire boundary (8 seconds) and to patches (30 seconds and later) after stimulation with 1 µM cAMP, whereas stimulation with 1 nM cAMP induces only patches of PHCrac-GFP (20 seconds and later).