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Fig. 4. Loss of triglucosylated Tyr(C85S) is unaffected by known protease inhibitors. (A) Pulse chase analysis of Tyr(C85S) or (B) wild-type Tyr in mouse melanocytes in the presence or absence of the indicated inhibitors throughout the time course, while an asterisk indicates the post-translational addition of the inhibitor. LP indicates leupeptin and pepstatin (10 µg/ml). (C) Human TYR(C89R) was translated in the presence of Tyr(C85S) SP-cells, with or without DNJ and/or ß-lac and separated on SDS-PAGE as previously described.





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