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Fig. 8. Aggregation of TYR(C89R) is inhibited by reducing agent and induced by non-hydrolyzable ATP. (A) TYR(C89R) was translated in the presence of SPTyr(C85S) melanocytes for 1 hour with or without DNJ or DTT. Membranes were isolated and resuspended in a proteasome-free buffer that contained DNJ, ATP-{gamma}-S or DTT. Samples were isolated at sequential time points and either separated on SDS-PAGE or precipitated with TCA (B).





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