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Fig. 3. F cells expressing the mutant L1106A exhibit delayed spreading on FN. (A) Serum-deprived F cells expressing vector only, WT, or the mutants L1106A or K634R were plated on FN-coated tissue culture plates in serum-free media and examined for their ability to spread 20, 40 and 90 minutes after plating. (B) F cell spreading-efficiency was quantified by calculating the percentage of spread cells at each time point. Shown is the mean±s.d. of triplicates from at least three independent experiments.