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Fig. 6. Immunofluorescence labeling of phosphorylated histone H3 and HTR12, and FISH detection of 180 bp repetitive sequences on mitotic metaphase chromosomes. (A-C) Polar view of chromosomes spread on the equatorial plate. (D-G) Equatorial view of chromosomes arranged on the equatorial plate. (A,E) FISH signals from 180 bp repeats (blue), (B,F) FISH signals from 180 bp repeats (blue) and immunosignals from phosphorylated histone H3 (green), (C,G) immunosignals from phosphorylated histone H3 (green) and from HTR12 (red). Chromosomes counterstained with DAPI appear gray (A-C) and pale gray (D). Scale bars: 2 µm.





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