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Fig. 4. Osteoblastic induction of MIAMI cells. (A) Alizarin Red-S staining of hydroxyapatite-associated calcium mineral deposited in the extracellular matrix by osteoblastic cells derived upon osteogenic induction (see Materials and Methods) of MIAMI cells. All plated cells induced to differentiate developed properties of functional osteoblasts. (B) RT-PCR analysis of transcripts present in osteoblast-induced MIAMI cells. MIAMI cells (lanes 1-4: different isolates) induced to differentiate to the osteoblastic lineage express the osteoblast phenotypic markers bone sialoprotein (BSP), osteocalcin (OC), runt-homology domain transcription factor Runx2, and osteopontin (OP). The size of the human-specific PCR products and the number of amplification cycles are in parentheses. BSP and OC transcripts were not detected in uninduced MIAMI cells (not shown).





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