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Fig. 6. Neural induction of MIAMI cells. Neural-competent cells require a sequential neuro-induction process of specification (step 1; bFGF), commitment (step 2; ßME/NT-3), and differentiation (step 3; NT-3/NGF/BDNF) (see Materials and Methods). Each stage is characterized by the stage-specific expression of specific markers. Nestin (top left) is expressed first, followed by induction of ß-III-tubulin (TuJ1) and NGF receptor (middle) in about 50% of the cells, and then expression of neurofilament-160 and NeuN (bottom) in nearly 40% of plated cells. Morphologically homogeneous cell populations are observed after each step.





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