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Fig. 5. Only the 309S mutant of CDC25B was distributed diffusely with co-transfection of 14-3-3ß. (A) Wild type CDC25B or different CDC25B mutants with a single phosphorylatable serine were cotransfected with 14-3-3ß into HEK293 cells. (Upper panels) Subcellular localization of CDC25B wild type and mutants. (Lower panel) Corresponding images of nuclei. (B) Percentage of cells transfected with mutant CDC25B 309S (shown in A) that express CDC25B with nuclear, diffuse and cytoplasmic distribution. Over 200 cells expressing CDC25B were counted to determine the percentage of cells that express CDC25B. Transfection with wild-type CDC25B alone (WT), 309S mutant of CDC25B alone (309S) and mutant 309S in combination with myc-tagged 14-3-3ß (309S +14-3-3ß) or myc-tagged 14-3-3{sigma} (309S +14-3-3{sigma}). Error bars in graphs represent the means±s.d. of three independent experiments. Subcellular distribution of CDC25B: N>C, predominantly nuclear; N=C diffuse; N<C, predominantly cytoplasmic (C).





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